Photoconvert linage tracking1/10/2023 ![]() With the near-infrared photoconvertible membrane dye, the entire visible spectral range is available for simultaneous use with other fluorescent proteins to monitor gene expression or to trace cell lineage commitment in vivo with high spatial and temporal resolution. We also studied the peripheral differentiation of individual T cells by tracking the gain or loss of FoxP3-GFP expression, a marker of the immune suppressive function of CD4(+) T cells. Combining the use of this photoconvertible dye with intravital microscopy, we tracked the division of individual hematopoietic stem/progenitor cells within the calvarium bone marrow of live mice. Ratiometric imaging can be used to distinguish photoconverted from non-converted cells with high sensitivity. We show that DiR exhibits a permanent fluorescence emission shift (photoconversion) after light exposure and does not reacquire the original color over time. Published by BMJ.We describe a novel photoconversion technique to track individual cells in vivo using a commercial lipophilic membrane dye, DiR. Our study combines in vivo lineage tracing and scRNA-seq to reveal the heterogeneity and dynamics of Prom1+ HCC cells, providing insights into the mechanistic role of malignant CSC-like cells in HCC progression. The activated oxidant detoxification underlies the protective mechanism of dedifferentiated transition and lineage propagation. Conserved gene signature of Prom1 linage predicts poor prognosis in human HCC. scRNA-seq analysis highlighted the heterogeneity of Prom1+ HCC cells, which follow a trajectory to the dedifferentiated status with high proliferation and stem cells traits. Here, we describe EosFPphotoconversion of Xenopus embryos to track cells during developmental and regenerative processes, using metal halide and xenon arc-based. Depletion of Prom1+ cells impedes tumour growth and reduces malignant cancer hallmarks in both HCC models. Neuronal tracing with DiI: decalcification, cryosectioning, and photoconversion for light and electron microscopic analysis. for cell tracking include the rapidity and stability of photoconversion. Labelled Prom1+ cells exhibit increasing tumourigenicity in 3D culture and allotransplantation, as well as potential to form cancers of differential lineages on transplantation. Tracing Cells for Tracking Cell Lineage and Clonal Behavior - Cell Press. Lineage tracing demonstrated that these cells display clonal expansion in situ in primary tumours. Prom1 in HCC tumours marks proliferative tumour-propagating cells with CSC-like properties. Single-cell RNA sequencing (scRNA-seq) was carried out to analyse the transcriptomic profile of traced Prom1+ cells. We performed lineage tracing post-HCC induction using Prom1 C-L/+ Rosa26 tdTomato/+ mice, and targeted depletion using Prom1 C-L/+ Rosa26 DTA/+ mice. We established two mouse models representing chronic fibrotic HCC and rapid steatosis-related HCC. The aim of this study was to investigate the heterogeneity and properties of Prom1+ cells in HCC in intact mouse models. We previously identified Prominin-1 (PROM1)/CD133 as an important liver cancer stem cell (CSC) marker in human HCC. To build an effective data lineage system, it is necessary to map the various data elements and the processes or algorithms they go. In recent years, new methods have emerged to enable cell lineage tracking with increasing resolution, leading to substantial biological insights (Woodworth et al., 2017). Using a Neo4j graph database to power your metadata management. Hepatocellular carcinoma (HCC) has high intratumoral heterogeneity, which contributes to therapeutic resistance and tumour recurrence. Resolving lineage relationships between cells is necessary to understand the fundamental mechanisms underlying normal development and the progression of disease.
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